In this assay, cells are plated in a 96 well format and treated with compounds are a range of concentrations. After an established amount of time, cells are fixed. Primary antibodies for the protein of interest and a housekeeping gene are added to each well. After overnight incubation, the primary antibodies are washed away and IR-labeled secondary antibodies are added. Plates are imaged and signal intensity is quantified using the Licor Scanner.
