For this assay, cells of interest were plated in a 96 well plate, as needed. Compounds were added to the cells in duplicate and at a range of concentrations. After 72hrs, we used Promega's CTG assay to measure % inhibition of cell growth. In this manner, we can read dozens of compounds to measure inhibition of cell proliferation by compounds of interest. In a similar manner, cell death and apoptosis can be measured using other commercially available kits. Below is a representative graph of the type of data that can be generated through this type of assay.
