This antibody was purified from a client supplied hybridoma, Once collected from the supernatant, the antibody was passed through a MabSelect column and dialyzed. The antibody was run on a 4-20% Bis-Tris denaturing gel and stained with Coomassie.
In addition to purification of antibodies from hybridomas, antibodies can also be produced in CHO cells, through transfection of plasmids containing the heavy and light chains.
